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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
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Genética
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JoVE Journal Genética
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

DOI:
10.3791/57609-v

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10:21 min

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October 07, 2018

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, , ,
1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM,Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition,Sorbonne Universités

Capítulos

  • 00:04Título
  • 00:40Collecting Fertilized Eggs from Superovulated Mice
  • 02:49Preparation of Injection Pipette Containing the Lentiviral Vector, Micro-Injection
  • 05:04Transferring Embryos into B6CBAF1/JRj Pseudopregnant Females
  • 08:10Results: Various Tested Constructs
  • 09:28Conclusion

Summary

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Here, we present a protocol to promote transgene integration and production of founder transgenic mice with high efficacy by a simple injection of a lentiviral vector in the perivitelline space of a fertilized oocyte.

Tags

LentiviralTransgenic MiceIn Vivo ScreeningGain-of-functionLoss-of-functionDNA Motif MappingOviductFertilized EggsHyaluronidaseCumulus CellsM16 MediumLentiviral VectorMicromanipulatorInjection PipetteHolding PipetteM2 MediumParaffin Oil

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