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电化学检测氘动力学同位素对希瓦氏菌 oneidensis MR-1 细胞外电子传输的影响

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Bioquímica

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JoVE Journal Bioquímica

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

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电化学检测氘动力学同位素对希瓦氏菌 oneidensis MR-1 细胞外电子传输的影响

DOI:

10.3791/57584-v

•

09:00 min

•

April 16, 2018

•

Yoshihide Tokunou, Kazuhito Hashimoto, Akihiro Okamoto

¹Department of Applied Chemistry,University of Tokyo, ²Global Research Center for Environment and Energy based on Nanomaterials Science,National Institute for Materials Science

Capítulos

00:05Título
01:00Formation of a Monolayer Biofilm of S. oneidensis MR-1 on an ITO Electrode
04:11Replacement of the Supernatant with Fresh DM Medium with 10 mM Lactate
05:24Addition of Deuterium Water to Measure the KIE on the EET Process
06:26Results: Microbial Current Change Induced by D₂O Addition and Confirmation of Rate-limitation by Extracellular Electron Transport Process
07:36Conclusion

Summary

Tadução automática

在这里, 我们提出了一个全细胞电化学实验的协议, 以研究质子传输的贡献, 通过外膜细胞色素复合体在希瓦氏菌 oneidensis MR-1 的细胞外电子转运率。

Tags

Keywords: Deuterium Kinetic Isotope Effect Extracellular Electron Transport Shewanella Oneidensis MR-1 Electrochemical Detection Proton Transport C-type Cytochromes Three-electrode Electrochemical Reactor ITO Substrate Anaerobic Conditions Lactate Yeast Extract

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