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一种研究人体 T 细胞召回反应的吸水泡协议

JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

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一种研究人体 T 细胞召回反应的吸水泡协议

DOI:

10.3791/57554-v

•

11:17 min

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August 11, 2018

•

Line L. Holm^2,3, Milica Vukmanovic-Stejic, Thomas Blauenfeldt, Thomas Benfield³, Peter Andersen, Arne N. Akbar, Morten Ruhwald

¹Department of Infectious Disease Immunology, Center for Vaccine Research,Statens Serum Institut, ²Department of Infectious Diseases,Hvidovre Hospital, ³Institute of Clinical Medicine, Faculty of Health and Medical Sciences,University of Copenhagen, ⁴Division of Infection and Immunity,University College London

Capítulos

00:00Título
01:31Tuberculin Skin Test
03:56Evaluation of Skin Reaction
07:24Harvest of Blister Fluid
08:35Resultados
10:12Conclusion

Summary

Tadução automática

在这里, 我们提供了一个示范的吸入水泡皮肤召回模型。该模型允许一个简单的访问研究人体自适应免疫反应, 例如在疫苗开发的背景下。

Tags

Keywords: Suction Blister T-cell Recall Response Adaptive Immunity PPD Tuberculosis BCG Vaccine Skin Test Intradermal Injection In Vivo Antigen-specific T-cells Cytokines

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