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Ex Utero Electroporation en Organotypic Slice culturen van muis embryonale hersenen voor Live-Imaging van GABAergic interneuronen migreren
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Neurociência
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JoVE Journal Neurociência
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
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Ex Utero Electroporation en Organotypic Slice culturen van muis embryonale hersenen voor Live-Imaging van GABAergic interneuronen migreren

DOI:
10.3791/57526-v

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09:50 min

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April 20, 2018

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, , ,
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience,Université de Montréal, 3Department of pathology and cellular biology,Université de Montréal, 4Department of Pediatrics,Université de Montréal

Capítulos

  • 00:04Título
  • 01:01Collection of Mouse Embryos
  • 02:51Intraventricular Plasmid Injections and Ex Vivo Electroporation of the MGE
  • 03:53Brain Dissection and Organotypic Slice Cultures
  • 07:16Results: Time-lapse Live-imaging of Electroporated INs in Organotypic Slices Cultured for 48 h.
  • 09:09Conclusion

Summary

Tadução automática

Tadução automática

Hier bieden we een goedkope en betrouwbare methode voor het genereren van electroporated brein organotypic segment culturen van muis embryo's geschikt voor confocale microscopie en live-imaging technieken.

Tags

Keywords: Ex Utero ElectroporationOrganotypic Slice CulturesEmbryonic Mouse BrainsLive-imagingMigrating GABAergic InterneuronsDevelopmental NeuroscienceGenetic Neurodevelopmental DisordersAutismEpilepsyBiosafety CabinetDissectionCultureNeural Based MediumTransfer PipetteBlack Wax

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