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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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JoVE Journal Ambiente
Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

13,534 Views

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10:14 min

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May 04, 2018

DOI:

10.3791/57390-v

DOI:
10.3791/57390-v

•

10:14 min
May 04, 2018

•

8 Views
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1Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service,United States Department of Agriculture, 2Horticultural Research Laboratory, Agricultural Research Service,United States Department of Agriculture

Summary

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This article demonstrates novel techniques developed for oral delivery of double-stranded RNA (dsRNA) through the vascular tissues of plants for RNA interference (RNAi) in phloem sap feeding insects.

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RNA InterferenceDsRNA DeliveryPhloemPlant-sap-feeding InsectsHemipteran PestsBrown Marmorated Stink BugOral DeliveryGene SilencingMolecular BiopesticidesCapillary ActionGreen Beans4th Instar Nymphs

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Cite this Article

Ghosh, S. K. B., Hunter, W. B., Park, A. L., Gundersen-Rindal, D. E. Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects. J. Vis. Exp. (135), e57390, doi:10.3791/57390 (2018).

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