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1Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Copenhagen, 2Department of Anesthesiology,Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine,Lund University
Capítulos
- 00:04Título
- 01:17Cannula Preparation
- 01:53Insertion of the Cannula into the Cisterna Magna for Chronic Injections of CSF Tracers
- 05:52Injection of CSF Tracers
- 06:43Injecting CSF Tracers in Anesthetized Mice with Chronic Cisterna Magna Cannulation
- 07:20Injecting CSF Tracers in Awake Mice with Chronic Cisterna Magna Cannulation
- 08:05Results: Analysis of CSF Tracer Distribution
- 09:02Conclusion
Aquí se describe un protocolo para realizar la canulación de cisterna magna (CMc), una forma mínimamente invasiva para ofrecer marcadores, sustratos y moléculas de señalización en el líquido cerebroespinal (CFS). Combinado con diferentes modalidades de imágenes, CMc permite sistema de glymphatic y evaluación dinámica de CSF, así como entrega de todo el cerebro de varios compuestos.
