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Biologia do Desenvolvimento
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The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens

DOI:
10.3791/57370-v

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08:57 min

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April 24, 2018

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, , , ,
1Department of Molecular Cell Biology,Leiden University Medical Center

Capítulos

  • 00:04Título
  • 00:51Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
  • 04:10Culture of EPDCs
  • 05:32Induction of Epithelial to Mesenchymal Transition in EPDCs
  • 06:31Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
  • 08:17Conclusion

Summary

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Tadução automática

The epicardium plays a crucial role in the development and repair of the heart by providing cells and growth factors to the myocardial wall. Here, we describe a method to culture human primary epicardial cells that enables the study and comparison of their developmental and adult characteristics.

Tags

Epicardial CellsPrimary CellsCell CultureEpithelial-mesenchymal TransitionCell IsolationTrypsin DissociationCell SuspensionCell Strainer

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