Journal
/
Genética
/
3 ' 快速扩增 CDNA 末端, 以图记录癌症
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Adapting 3′ Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
Please note that all translations are automatically generated. Click here for the English version.

3 ' 快速扩增 CDNA 末端, 以图记录癌症

12,075 Views

•

09:38 min

•

March 28, 2018

DOI:

10.3791/57318-v

DOI:
10.3791/57318-v

•

09:38 min
March 28, 2018

•

2 Views
,
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences,Butler University

Summary

Automatically generated

在这里描述的两种不同的 3 ' 快速扩增的 cDNA 结束 (3 ' 种族) 协议使用两种不同的 DNA 聚合酶来映射序列, 包括开放阅读框架 (ORF) 的一部分, 停止密码子, 和整个 3 ' UTR 的成绩单使用 RNA从不同的癌细胞系获得的。

Explore More Videos

3′ RACEMRNA TranscriptsCancerNested PCRTranscript SequencingPolyadenylation SignalStop Codon3′ UTRFusion GenesSplice VariantsRNA ExtractionPhenol-chloroformRNA PrecipitationRNA Resuspension

Read Article

Cite this Article

Masamha, C. P., Todd, Z. Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer. J. Vis. Exp. (133), e57318, doi:10.3791/57318 (2018).

Download .ris file

Copy

Share Video

.

Copy