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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
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光遗传学与人工 microRNAs 相结合对完整神经元回路中基因击倒对突触前功能的影响

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09:17 min

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March 14, 2018

DOI:

10.3791/57223-v

DOI:
10.3791/57223-v

•

09:17 min
March 14, 2018

•

2 Views
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1Center for Synaptic Neuroscience and Technology,Istituto Italiano di Tecnologia

Summary

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该协议提供了一个工作流, 如何将人工 microRNA 介导的 RNA 干扰与光遗传学相结合, 以刺激特定突触前 boutons 在完整的神经元回路中的选择性基因表达减少。

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OptogeneticsArtificial MicroRNARNA InterferencePresynaptic FunctionNeuronal CircuitsSynaptic PhysiologyPresynaptic ProteinsNeuronal CultureGene KnockdownAcute Brain SlicesIn VivoCorticoculturingAraCRNA ExtractionChloroform

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Cite this Article

Thalhammer, A., Jaudon, F., Cingolani, L. A. Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits. J. Vis. Exp. (133), e57223, doi:10.3791/57223 (2018).

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