Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Agnes Thalhammer; Fanny Jaudon; Lorenzo A. Cingolani

doi:10.3791/57223

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Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

JoVE Journal
神経科学

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JoVE Journal 神経科学

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

9,932 Views

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09:17 min

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March 14, 2018

DOI:

10.3791/57223-v

DOI:

10.3791/57223-v

•

09:17 min

March 14, 2018

•

2 Views

Agnes Thalhammer¹, Fanny Jaudon¹, Lorenzo A. Cingolani¹

¹Center for Synaptic Neuroscience and Technology,Istituto Italiano di Tecnologia

概要

This protocol provides a workflow on how to combine artificial microRNA-mediated RNA interference with optogenetics to stimulate specifically presynaptic boutons with reduced expression of selective gene(s) within intact neuronal circuits.

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Optogenetics Artificial MicroRNA RNA Interference Presynaptic Function Neuronal Circuits Synaptic Physiology Presynaptic Proteins Neuronal Culture Gene Knockdown Acute Brain Slices In Vivo Corticoculturing AraC RNA Extraction Chloroform

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Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

概要

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