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大动物淋巴结的采集与处理 RNA 分析: 大动物 Transcriptomic 淋巴结的制备研究
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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大动物淋巴结的采集与处理 RNA 分析: 大动物 Transcriptomic 淋巴结的制备研究

DOI:
10.3791/57195-v

•

12:53 min

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May 19, 2018

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, , ,
1National Animal Disease Center, Agricultural Research Service,U.S. Department of Agriculture

Capítulos

  • 00:00Título
  • 01:52Collection of supplies
  • 03:18Methods
  • 09:41Resultados
  • 12:05Summary

Summary

Tadução automática

Tadução automática

本议定书概述了从大型动物的淋巴结组织 transcriptomic 分析中分离 RNA 的程序, 包括从家畜和野生动物中鉴别和切除淋巴结的步骤, 取样方法为了在多个动物之间提供一致性, 并考虑到收集后保存和处理 RNA 分析的代表性结果。

Tags

Keywords: Lymph NodesRNA AnalysisLarge AnimalsTranscriptomic StudiesZoonotic DiseasesBrucellosisE. Coli O157:H7DeerElkRNA PreservationRNAlaterNecropsyVeterinary ResearchBiologia Molecular

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