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Twee-foton Intravital Imaging van leukocyten tijdens de immuunrespons bij Lipopolysaccharide-behandelde muis lever
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
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Twee-foton Intravital Imaging van leukocyten tijdens de immuunrespons bij Lipopolysaccharide-behandelde muis lever

9,917 Views

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07:06 min

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February 06, 2018

DOI:

10.3791/57191-v

DOI:
10.3791/57191-v

•

07:06 min
February 06, 2018

•

14 Views
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1Department of Anatomy,Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science,Yonsei University College of Medicine, 3Department of Surgery,Yonsei University College of Medicine

Summary

Automatically generated

We een nieuwe chirurgische protocol voor twee-foton beeldvorming van levende muizen lever met minimale invasie opgericht. Met deze techniek vastgesteld we de gedetailleerde structuur van de lever tijdens lipopolysaccharide-geïnduceerde endotoxemia. Wij verwachten dat deze methode kan worden gebruikt om te bepalen van de effectiviteit van verschillende behandeling van de reagentia te hepatische migratie.

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Cite this Article

Park, S. A., Choe, Y. H., Lee, S. H., Hyun, Y. Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver. J. Vis. Exp. (132), e57191, doi:10.3791/57191 (2018).

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