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用于原位晶体 x 射线衍射和原位的微流控芯片系列晶体学的动态光散射
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11:48 min
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April 24, 2018
DOI:
1Center for Free Electron Laser Science,DESY, 2Department of Physics,University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation,University of Hamburg, 4The Hamburg Center for Ultrafast Imaging,University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry
Summary
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本协议详细描述了如何制造和操作微流控器件, 用于 X 射线衍射数据的室温采集。此外, 还介绍了如何通过动态光散射监测蛋白质结晶, 以及如何处理和分析获得的衍射数据。
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Cite this Article
Gicquel, Y., Schubert, R., Kapis, S., Bourenkov, G., Schneider, T., Perbandt, M., Betzel, C., Chapman, H. N., Heymann, M. Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography. J. Vis. Exp. (134), e57133, doi:10.3791/57133 (2018).
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