Journal
/
Medicina
/
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

DOI:
10.3791/57079-v

•

10:35 min

•

April 05, 2018

•

, , ,
1Biodesix

Capítulos

  • 00:04Título
  • 01:13Reverse Transcription of RNA to cDNA
  • 02:23Digital PCR
  • 05:29Data Analysis and Review and Generation of Results
  • 07:27Resultados
  • 09:23Conclusion

Summary

Tadução automática

Tadução automática

Detection of circulating ribonucleic acid (cRNA) from blood is an unmet need in clinical diagnostics. Here we describe methods that characterize cRNA from non-small cell lung cancer patients using sensitive and specific digital polymerase chain reaction. The tests meet design requirements to detect fusion variants within 72 hours.

Tags

Keywords: Blood-based TestROS1RETFusion TranscriptsCirculating Ribonucleic AcidDigital Polymerase Chain ReactionTumor-derived RNAOncology DiagnosticsMinimally InvasiveNSCLCReverse TranscriptionCDNAPCRFluorescent Probes

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57079?article_type=v

The Trier Social Stress Test Protocol for Inducing Psychological Stress
check_url/pt/57079?article_type=v

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
check_url/pt/57079?article_type=v

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring
check_url/pt/57079?article_type=v

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
check_url/pt/57079?article_type=v

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
check_url/pt/57079?article_type=v

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
check_url/pt/57079?article_type=v

Taste Exam: A Brief and Validated Test
check_url/pt/57079?article_type=v

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
check_url/pt/57079?article_type=v

Traditional Trail Making Test Modified into Brand-new Assessment Tools: Digital and Walking Trail Making Test
check_url/pt/57079?article_type=v

Psychophysical Tracking Method to Assess Taste Detection Thresholds in Children, Adolescents, and Adults: The Taste Detection Threshold (TDT) Test

Read Article