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A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
JoVE Journal
Medicine
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JoVE Journal Medicine
A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

English

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10,023 Views

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10:35 min

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April 05, 2018

DOI:

10.3791/57079-v

DOI:
10.3791/57079-v

•

10:35 min
April 05, 2018

•

5 Views
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1Biodesix

Summary

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Detection of circulating ribonucleic acid (cRNA) from blood is an unmet need in clinical diagnostics. Here we describe methods that characterize cRNA from non-small cell lung cancer patients using sensitive and specific digital polymerase chain reaction. The tests meet design requirements to detect fusion variants within 72 hours.

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Cite this Article

Mellert, H. S., Alexander, K. E., Jackson, L. P., Pestano, G. A. A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction. J. Vis. Exp. (134), e57079, doi:10.3791/57079 (2018).

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