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Trichostatin a 和维生素 C 的组合治疗提高体细胞细胞核移植克隆小鼠的效率
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April 26, 2018
DOI:
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology,Kindai University, 2Faculty of Biology-Oriented Science and Technology,Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology,University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture,Kyoto University, 5Institute of Advanced Technology,Kindai University
Summary
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我们用 trichostatin a、维生素 C 和去离子牛血清白蛋白来描述一种显著改进的小鼠克隆方法。我们展示了一个简化的、可重现的协议, 支持克隆胚胎的高效开发。因此, 该方法可以成为鼠标克隆的标准化程序。
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Azuma, R., Miyamoto, K., Oikawa, M., Yamada, M., Anzai, M. Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer. J. Vis. Exp. (134), e57036, doi:10.3791/57036 (2018).
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