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Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
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Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
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April 26, 2018
DOI:
1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology,Kindai University, 2Faculty of Biology-Oriented Science and Technology,Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology,University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture,Kyoto University, 5Institute of Advanced Technology,Kindai University
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We describe a dramatically improved method for mouse cloning using trichostatin A, vitamin C, and deionized bovine serum albumin. We show a simplified, reproducible protocol that supports efficient development of cloned embryos. Hence, this method could become a standardized procedure for mouse cloning.
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Azuma, R., Miyamoto, K., Oikawa, M., Yamada, M., Anzai, M. Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer. J. Vis. Exp. (134), e57036, doi:10.3791/57036 (2018).
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