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Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

6,694 Views

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10:56 min

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December 21, 2017

DOI:

10.3791/56936-v

DOI:
10.3791/56936-v

•

10:56 min
December 21, 2017

•

4 Views
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1Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Associazione Annastaccatolisa Onlus, 3Nuclear Medicine Unit,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

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We present a new approach to characterize tumor cells. We combined immunofluorescence with DNA fluorescent-in-situ-hybridization to evaluate cells captured by a functionalized medical wire capable of in vivo enriching CTCs directly from patient blood.

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Gallerani, G., Cocchi, C., Bocchini, M., Piccinini, F., Fabbri, F. Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH. J. Vis. Exp. (130), e56936, doi:10.3791/56936 (2017).

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