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成人皮肤角质形成细胞的生成与培养
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
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成人皮肤角质形成细胞的生成与培养

DOI:
10.3791/56863-v

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10:42 min

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December 22, 2017

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1Department of Dermatology,Aarhus University Hospital

Capítulos

  • 00:05Título
  • 00:52Isolation of Keratinocytes from Human Skin
  • 05:32Passaging of Keratinocytes
  • 07:32Freezing Keratinocytes
  • 08:48Results: Primary Human Keratinocyte Culture from Adult Skin
  • 10:06Conclusion

Summary

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Tadução automática

人类皮肤作为抵御外部环境的第一条防线。我们提出了一种从成人皮肤中分离原代角质形成细胞的方法。这些孤立的角质形成细胞是有用的许多实验设置, 是一个非常适合的模型研究的分子机制在皮肤生物学体外.

Tags

Primary Human KeratinocytesAdult SkinEpidermal Cell BiologySkin DiseasesTrypsinDPBSKSFMRPMI 1640FBSGentamicinEpidermal CellsDermal Compartment

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