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En roman In Vitro sår Healing Assay for at evaluere celle Migration
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

En roman In Vitro sår Healing Assay for at evaluere celle Migration

DOI:
10.3791/56825-v

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08:55 min

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March 17, 2018

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, ,
1Department of Biochemical Sciences,Sapienza University of Rome

Capítulos

  • 00:00Título
  • 01:28Cell Preparation
  • 03:34Cell Seeding in the Culture Inserts
  • 04:30Pseudo-wound Healing Assay
  • 05:52Image Analysis
  • 06:53Representative Results
  • 07:34Effects of Inhibitors on Cells Migration
  • 08:33Conclusions

Summary

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Vi præsenterer her, en protokol for at vurdere effekten af peptider for migration af bronchiale epitel celler. Denne metode giver mulighed for hurtig og meget reproducerbare opnåelse af kvantitative data om hastighed i celle migration og sår lukning.

Tags

Keywords: Cell MigrationWound HealingIn Vitro AssayImmunomodulatory MoleculesAntimicropeptidesCell MonolayerPseudo-woundImage AnalysisBronchial Epithelial CellsEpidermal Growth Factor Receptor

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