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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

DOI:
10.3791/56825-v

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08:55 min

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March 17, 2018

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, ,
1Department of Biochemical Sciences,Sapienza University of Rome

Capítulos

  • 00:00Título
  • 01:28Cell Preparation
  • 03:34Cell Seeding in the Culture Inserts
  • 04:30Pseudo-wound Healing Assay
  • 05:52Image Analysis
  • 06:53Representative Results
  • 07:34Effects of Inhibitors on Cells Migration
  • 08:33Conclusions

Summary

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Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound closure.

Tags

Keywords: Cell MigrationWound HealingIn Vitro AssayImmunomodulatory MoleculesAntimicropeptidesCell MonolayerPseudo-woundImage AnalysisBronchial Epithelial CellsEpidermal Growth Factor Receptor

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