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Eksperimentel protokol til påvisning af mitokondriel funktion i hepatocytter udsat for organochlorpe pesticider
1State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine
Capítulos
- 00:04Título
- 00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
- 02:45Mitochondrial Membrane Potential Measurements
- 04:39Oxygen Consumption Rate (OCR) Measurements
- 06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
- 07:43Conclusion
Forståelse af indflydelsen af miljømæssige organochlorin pesticider (OCPs) på mitokondriel funktion i hepatocytes er vigtigt at udforske mekanismen af OCPs forårsager metaboliske lidelser. Dette papir præsenterer detaljerede metoder til påvisning af hepatisk mitokondriel funktion.
