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Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides
 

Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

Article DOI: 10.3791/56800-v • 08:39 min • September 16th, 2020
September 16th, 2020 •
1,2,3, 3, 1,2
1State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease, Tongji University School of Medicine

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Understanding the influence of environmental organochlorine pesticides (OCPs) on mitochondrial function in hepatocytes is important in exploring the mechanism of OCPs causing metabolic disorders. This paper presents detailed methods on detecting hepatic mitochondrial function.

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Experimental Protocol Detecting Mitochondrial Function Hepatocytes Organochlorine Pesticides ATP Level Oxygen Consumption Rates Glass Bottom Petri Dish Beta-hexachlorcyclohexane Mitochondrial Green Fluorescence Probe Solution Confocal Microscope Cellular ATP Level Lysis Buffer
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