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In Vivo Overvågning af døgnrytmen ur genekspression i mus Suprachiasmatic kernen bruger fluorescens journalister
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Neurociência
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JoVE Journal Neurociência
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Overvågning af døgnrytmen ur genekspression i mus Suprachiasmatic kernen bruger fluorescens journalister

DOI:
10.3791/56765-v

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07:44 min

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July 04, 2018

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, ,
1PTN Joint Graduate Program, School of Life Sciences,Peking University, 2National Institute of Biological Sciences, Beijing

Capítulos

  • 00:04Título
  • 00:37Preparation of Optical Fiber
  • 01:14rAAV Cry1 Reporter Injection
  • 03:21Optic Fiber Insertion into the Adult Mouse SCN
  • 04:05In Vivo Fluorescence Signal Monitoring and Data Collection
  • 05:24Results: Flourescence In Vivo Recording in the SCN Under LD and DD Conditions
  • 06:54Conclusion

Summary

Tadução automática

Tadução automática

Dette nyudviklede fluorescens-baseret teknologi gør det muligt for langsigtet overvågning af transkription af døgnrytmen ur gener i suprachiasmatic kernen (SCN) af frit flytte mus i realtid og med en høj tidsmæssige opløsning.

Tags

Keywords: In Vivo MonitoringCircadian Clock Gene ExpressionMouse Suprachiasmatic NucleusFluorescence ReportersOptical FiberRecombinant AAVStereotaxic InjectionSCN

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