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In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
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Neurociência
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JoVE Journal Neurociência
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

DOI:
10.3791/56765-v

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07:44 min

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July 04, 2018

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, ,
1PTN Joint Graduate Program, School of Life Sciences,Peking University, 2National Institute of Biological Sciences, Beijing

Capítulos

  • 00:04Título
  • 00:37Preparation of Optical Fiber
  • 01:14rAAV Cry1 Reporter Injection
  • 03:21Optic Fiber Insertion into the Adult Mouse SCN
  • 04:05In Vivo Fluorescence Signal Monitoring and Data Collection
  • 05:24Results: Flourescence In Vivo Recording in the SCN Under LD and DD Conditions
  • 06:54Conclusion

Summary

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Tadução automática

This newly developed fluorescence-based technology enables long-term monitoring of the transcription of circadian clock genes in the suprachiasmatic nucleus (SCN) of freely moving mice in real-time and at a high temporal resolution.

Tags

Keywords: In Vivo MonitoringCircadian Clock Gene ExpressionMouse Suprachiasmatic NucleusFluorescence ReportersOptical FiberRecombinant AAVStereotaxic InjectionSCN

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