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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

12,159 Views

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12:02 min

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May 02, 2018

DOI:

10.3791/56730-v

DOI:
10.3791/56730-v

•

12:02 min
May 02, 2018

•

15 Views
, ,
1Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health,Centers for Disease Control and Prevention

Summary

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Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.

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Cite this Article

Lemons, A. R., Lindsley, W. G., Green, B. J. Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA. J. Vis. Exp. (135), e56730, doi:10.3791/56730 (2018).

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