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Immunology and Infection

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JoVE Journal Immunology and Infection
Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes
 

Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes

Article DOI: 10.3791/56685-v • 08:30 min • March 15th, 2018
March 15th, 2018 •
1, 1, 1,2, 1, 2, 3, 2, 1,2
1Translational Experimental Pediatrics, Experimental Pulmonology, Department of Pediatrics and Adolescent Medicine, University of Cologne, 2Department of Pediatrics and Adolescent Medicine, University of Cologne, 3Division of Experimental Pediatrics and Metabolism, University Children's Hospital, Heinrich Heine University Düsseldorf

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The incidence of obesity is rising and increases the risk of chronic lung diseases. To establish the underlying mechanisms and preventive strategies, well-defined animal models are needed. Here, we provide three methods (glucose-tolerance-test, body plethysmography, and lung fixation) to study the effect of obesity on pulmonary outcomes in mice.

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Intraperitoneal Glucose Tolerance Test Measurement Of Lung Function Fixation Of The Lung Impact Of Obesity Impaired Metabolism Pulmonary Outcomes Respiratory Research Metabolic Disorders Lung Structure Lung Function Chronic Lung Diseases Therapeutical Approaches Phenotyping Metabolic Targets Animal Models COPD Asthma Tracheal Tube Insertion Insufflation Of The Lung Deep Anesthesia Administration Early Onset Obesity Intraperitoneal Glucose Tolerance Measurement
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