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Auditive hjernestammen svar og ydre hår celle hele-celle Patch klemme optagelse i Postnatal rotter
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Auditive hjernestammen svar og ydre hår celle hele-celle Patch klemme optagelse i Postnatal rotter
1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University
Capítulos
- 00:04Título
- 00:56Auditory Brainstem Response Recording
- 02:12The Organ of Corti Dissection
- 03:22Immunofluorescence Staining
- 05:21Patch Clamp Recording of Isolated OHCs
- 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
- 08:19Conclusion
Denne protokol beskriver metoder til at registrere den auditive hjernestammen svar fra postnatal rotte unger. For at undersøge den funktionelle udvikling af ydre hårceller, er forsøgsmetoden af hele-celle patch klemme optagelse i isolerede ydre hårceller beskrevet trin for trin.
