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מיפוי בו זמנית, כימות של Ribonucleotides ב- DNA מיטוכונדריאלי אנושי
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Biologia
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JoVE Journal Biologia
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
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מיפוי בו זמנית, כימות של Ribonucleotides ב- DNA מיטוכונדריאלי אנושי

9,225 Views

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12:35 min

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November 14, 2017

DOI:

10.3791/56551-v

DOI:
10.3791/56551-v

•

12:35 min
November 14, 2017

•

2 Views
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1Department for Medical Biochemistry and Cell Biology,University of Gothenburg, 2Department of Biology and Biological Engineering,Chalmers University of Technology

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Mitochondrial DNARibonucleotidesDNA IntegrityDNA ReplicationDNA RepairHeLa CellsHincIIParamagnetic Beads70% EthanolPotassium HydroxidePotassium ChlorideSodium Acetate

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Kreisel, K., Engqvist, M. K., Clausen, A. R. Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA. J. Vis. Exp. (129), e56551, doi:10.3791/56551 (2017).

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