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Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
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November 14, 2017
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Here we describe a method amenable to simultaneously quantitate and genome-wide map ribonucleotides in highly intact DNA at single-nucleotide resolution, combining enzymatic cleavage of genomic DNA with its alkaline hydrolysis and subsequent 5´-end sequencing.
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Kreisel, K., Engqvist, M. K., Clausen, A. R. Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA. J. Vis. Exp. (129), e56551, doi:10.3791/56551 (2017).
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