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应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

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应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞

11,204 Views

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12:34 min

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December 06, 2017

DOI:

10.3791/56538-v

DOI:

10.3791/56538-v

•

12:34 min

December 06, 2017

•

6 Views

Asa S. Hidmark¹, Peter P. Nawroth^1,2,3, Thomas Fleming^1,3

¹Department of Medicine I and Clinical Chemistry,University Hospital of Heidelberg, ²Institute for Diabetes and Cancer IDC Helmholtz Center Munich,Germany & Joint Heidelberg-IDC Translational Diabetes Program, ³German Center for Diabetes Research (DZD)

Summary

由于组织的缺乏, 小鼠坐骨神经内细胞含量的定量分析是困难的。本协议描述了一种组织消化和制备的方法, 它提供了足够的细胞, 用于对单个小鼠神经的免疫细胞进行流式细胞仪分析。

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