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JoVE Journal Developmental Biology
Una base de RANKL osteoclasto cultura ensayo de médula ósea de ratón para investigar el papel de mTORC1 en la formación de osteoclastos
 
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Una base de RANKL osteoclasto cultura ensayo de médula ósea de ratón para investigar el papel de mTORC1 en la formación de osteoclastos

Article DOI: 10.3791/56468-v • 09:37 min • March 15th, 2018
March 15th, 2018 •
*1, *2, *1, 3, 2, 1, 2, 1
1Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 2State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, 3Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine
* These authors contributed equally

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Este manuscrito describe un protocolo para aislar y la cultura osteoclastos en vitro de médula ósea de ratón y para estudiar el papel de lo mamíferos/mecanicista Diana de la rapamicina 1 complejo en la formación de osteoclastos.

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Biología del desarrollo cuestión 133 osteoclastos mTORC1 Raptor hueso remodelación biología celular RANKL M-CSF
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