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共焦点顕微鏡を用いた可視化マクロファージ細胞外トラップ

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

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共焦点顕微鏡を用いた可視化マクロファージ細胞外トラップ

9,850 Views

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09:14 min

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October 19, 2017

DOI:

10.3791/56459-v

DOI:

10.3791/56459-v

•

09:14 min

October 19, 2017

•

18 Views

Roleen Sharma², Kim M. O’Sullivan, Stephen R. Holdsworth, Philip G. Bardin², Paul T. King²

¹Monash Lung and Sleep,Monash Medical Centre, ²Monash University

Tags

Macrophage Extracellular Traps Confocal Microscopy Inflammation Bronchoalveolar Lavage Trypan Blue Hemocytometer Paraformaldehyde-periodate-lysine Fixative TWEEN 20 Chicken Serum BSA Primary Antibodies Secondary Antibodies DAPI FFPE Samples Antigen Retrieval Pressure Cooker Tris-EDTA

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