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Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
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JoVE 杂志 免疫与感染
Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

DOI:
10.3791/56459-v

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09:14 min

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October 19, 2017

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, , , ,
1Monash Lung and Sleep,Monash Medical Centre, 2Monash University

Chapters

  • 00:05Title
  • 00:46Collect, Fluorescently Label, and Image Bronchoalveolar Lavage Macrophages
  • 02:50Preparation/Microscopy of Lung Tissue Samples
  • 05:35Three-dimensional Imaging of METs
  • 06:54Results: Macrophage Extracellular Trap Visualization
  • 08:16Conclusion

Summary

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Macrophage extracellular traps are a newly described entity. This article will concentrate on confocal microscopy methods and how they are visualized in vitro and in vivo from lung samples.

Tags

Macrophage Extracellular TrapsConfocal MicroscopyInflammationBronchoalveolar LavageTrypan BlueHemocytometerParaformaldehyde-periodate-lysine FixativeTWEEN 20Chicken SerumBSAPrimary AntibodiesSecondary AntibodiesDAPIFFPE SamplesAntigen RetrievalPressure CookerTris-EDTA

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