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利用共焦显微镜可视化巨噬细胞胞外陷阱
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
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利用共焦显微镜可视化巨噬细胞胞外陷阱

9,849 Views

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09:14 min

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October 19, 2017

DOI:

10.3791/56459-v

DOI:
10.3791/56459-v

•

09:14 min
October 19, 2017

•

18 Views
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1Monash Lung and Sleep,Monash Medical Centre, 2Monash University

Tags

Macrophage Extracellular TrapsConfocal MicroscopyInflammationBronchoalveolar LavageTrypan BlueHemocytometerParaformaldehyde-periodate-lysine FixativeTWEEN 20Chicken SerumBSAPrimary AntibodiesSecondary AntibodiesDAPIFFPE SamplesAntigen RetrievalPressure CookerTris-EDTA

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Sharma, R., O'Sullivan, K. M., Holdsworth, S. R., Bardin, P. G., King, P. T. Visualizing Macrophage Extracellular Traps Using Confocal Microscopy. J. Vis. Exp. (128), e56459, doi:10.3791/56459 (2017).

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