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Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
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Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Capítulos
- 00:05Título
- 00:45Cells and Biotinylated CPPs
- 02:01Cellular Treatment with the BCPPs
- 03:49Protein Extraction and Pull-down
- 07:19Results: Study of the Intracellular TAT-Cx43266-283-B Interactions in G166 GSCs
- 09:00Conclusion
This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.
