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1School of Public Health of Southeast University,Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering,Southeast University, 3School of Public Health,Tianjin Medical University, 4British Columbia Academy,Nanjing Foreign Language School
Capítulos
- 00:04Título
- 01:27Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
- 04:44Preparation of Real Urine Samples and Mobile Phase
- 06:25PFSPE Extraction and HPLC Analysis
- 09:58Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
- 11:43Conclusion
Presentamos una cómoda extracción en fase sólida acoplada a cromatografía líquida de alta presión (HPLC) con detección electroquímica (ECD) para la determinación simultánea de los tres neurotransmisores monoamina y dos de sus metabolitos en la orina de los bebés. También identificamos el metabolito MHPG como un potencial biomarcador para el diagnóstico precoz de daño cerebral para los niños.
