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Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus
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Neurociência
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JoVE Journal Neurociência
Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

DOI:
10.3791/56438-v

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09:59 min

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October 11, 2017

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, , , , , ,
1Center for Functional Connectomics,Korea Institute of Science and Technology, 2Biomedical department, KIST school,University of Science and Technology, 3Department of Biological Sciences,Korea Advanced Institute of Science and Technology

Capítulos

  • 00:05Título
  • 00:59Preparation of the Probe
  • 02:28Fixation of a 3D-printed Hat to the Skull
  • 05:01Implantation of the Silicon Probe
  • 07:40Probe Recovery
  • 08:30Results: Chronic Recording of Place Cells
  • 09:14Conclusion

Summary

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We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.

Tags

ImplantationSilicon ProbesHippocampal Place CellsEnriched TreadmillMouseAnesthesiaSurgeryElectrophysiologyRecordingNeural ActivityBregmaLambdaStereotaxicManual StepsBinocular MicroscopeDental CementDetergentCleaningIsofluraneBuprenorphineAnalgesiaHemostasis

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