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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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Genética
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JoVE Journal Genética
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

DOI:
10.3791/56423-v

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07:03 min

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January 02, 2018

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, ,
1Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,University of California, Los Angeles

Capítulos

  • 00:05Título
  • 00:38ActD Time Course
  • 02:13Isolation of Total RNA from Phenol-guanidine Isothiocyanate Solution Lysate
  • 05:16Analysis of Transcript Abundance
  • 06:02Results: Clear Contrast in Transcript Decay Between Proliferating and Quiescent Fibroblasts
  • 06:28Conclusion

Summary

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We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.

Tags

Keywords: Genome-wideTranscriçãoDecay RatesProliferatingQuiescentFibroblastsBiologia MolecularGene ExpressionTranscript AbundanceActDPBSPhenol-guanidine IsothiocyanateSpike-inChloroform

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