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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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Genetik
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JoVE Journal Genetik
Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

6,094 Views

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07:03 min

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January 02, 2018

DOI:

10.3791/56423-v

DOI:
10.3791/56423-v

•

07:03 min
January 02, 2018

•

9 Views
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1Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles, 2Department of Biological Chemistry,David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program,University of California, Los Angeles

Summary

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We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.

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Genome-wideTranskriptDecay RatesProliferatingQuiescentFibroblastsMolekularbiologieGene ExpressionTranscript AbundanceActDPBSPhenol-guanidine IsothiocyanateSpike-inChloroform

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Mitra, M., Lee, H. N., Coller, H. A. Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts. J. Vis. Exp. (131), e56423, doi:10.3791/56423 (2018).

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