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Generation af en gen-forstyrret Streptococcus mutans stamme uden Gene kloning
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12:06 min
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October 23, 2017
DOI:
1Department of Translational Research,Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research),Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University
Summary
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Vi beskriver en letkøbt, hurtig og relativt billig metode for generation af gen-forstyrret Streptococcus mutans stammer; denne teknik kan tilpasses til generation af gen-forstyrret stammer af forskellige arter.
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Murata, T., Okada, A., Matin, K., Hanada, N. Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning. J. Vis. Exp. (128), e56319, doi:10.3791/56319 (2017).
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