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Multi foton Time Lapse Imaging te visualiseren van ontwikkeling in realtime: visualisatie van migreren neurale kamcellen in Zebrafish embryo's
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Capítulos
- 00:05Título
- 00:48Embryo Collection for Time-lapse Imaging
- 01:51Embryo Preparation
- 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
- 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
- 07:26Post-acquisition Processing
- 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
- 09:29Conclusion
Een combinatie van de geavanceerde optische technieken van laser scanning microscopie met lange golflengte fluorescentie multi foton excitatie werd uitgevoerd om vast te leggen van real-time, hoge resolutie, driedimensionale beeldvorming van neurale crest migratie in GS (sox10:EGFP) en GS (foxd3:GFP) zebrafish embryo's.
