Journal
/
Biologia do Desenvolvimento
/
多光子时间推移成像实时可视化发展： 迁移神经嵴细胞在斑马鱼胚胎中的可视化
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
Please note that all translations are automatically generated. Click here for the English version.

多光子时间推移成像实时可视化发展： 迁移神经嵴细胞在斑马鱼胚胎中的可视化

DOI:
10.3791/56214-v

•

10:13 min

•

August 09, 2017

•

,
1Department of Ophthalmology and Visual Sciences, Kellogg Eye Center,University of Michigan

Capítulos

  • 00:05Título
  • 00:48Embryo Collection for Time-lapse Imaging
  • 01:51Embryo Preparation
  • 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
  • 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
  • 07:26Post-acquisition Processing
  • 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
  • 09:29Conclusion

Summary

Tadução automática

Tadução automática

激光扫描与波长多光子荧光显微镜的先进光学技术组合的实施是为了捕获高分辨率的、 三维的、 实时成像中甘油三酯 (sox10:EGFP) 和甘油三酯 (foxd3:GFP) 斑马鱼胚胎神经嵴迁移。

Tags

Multi-photon Time Lapse ImagingZebrafish EmbryosNeural Crest Cell MigrationBiologia do DesenvolvimentoCell Migration VisualizationConfocal MicroscopyMulti-photon LaserEmbryo CollectionTransgenic EmbryosGFPPTULowMelt AgaroseOpen Bath Chamber

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

分析<em>在体内</em>细胞迁移使用细胞移植和时间推移成像在斑马鱼胚胎

配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析

体细胞重编程的动力学测量和实时可视化

诱导生活青蛙和斑马鱼胚胎缺氧

定量造血干和祖细胞 (HSPCs) 在斑马鱼胚胎发育中的体外试验研究

P2X7 受体实时活细胞流式细胞仪检测成人神经祖细胞中的钙影响、毛孔形成和吞噬作用

全斑马鱼胚胎的延长时移共聚焦显微镜的分层安装方法

使用"彩虹"和"延时共聚焦成像"可视化活斑马鱼中发育中的脑

神经冠状原脂肪衍生干细胞的分离、培养和异位诱导，来自围阴脂肪组织

CCL5诱导小鼠骨膜骨骼干细胞迁移的实时成像

Read Article