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Probenvorbereitung und Analyse der RNASeq-basierte Genexpressionsdaten von Zebrafisch
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Genética
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JoVE Journal Genética
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Probenvorbereitung und Analyse der RNASeq-basierte Genexpressionsdaten von Zebrafisch

10,482 Views

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11:42 min

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October 27, 2017

DOI:

10.3791/56187-v

DOI:
10.3791/56187-v

•

11:42 min
October 27, 2017

•

3 Views
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1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine,University of Maryland School of Medicine

Summary

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Dieses Protokoll stellt einen Ansatz für die ganze Transkriptom-Analyse von Zebrafisch-Embryonen, Larven, oder Zellen sortiert. Wir gehören Isolierung von RNA, Pathway-Analyse von RNASeq Daten und qRT-PCR-basierte Validierung von Veränderungen der Genexpression.

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RNASeqGene ExpressionZebrafishEmbryoLarvaTranscriptomeSample PreparationRNA ExtractionRNA SequencingBiologia do DesenvolvimentoComparative Analysis

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Cite this Article

Hostelley, T. L., Nesmith, J. E., Zaghloul, N. A. Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish. J. Vis. Exp. (128), e56187, doi:10.3791/56187 (2017).

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