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Atlas-guida stereotassica del Laser Capture Microdissection di regioni del cervello colpite da lesione traumatica
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
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Atlas-guida stereotassica del Laser Capture Microdissection di regioni del cervello colpite da lesione traumatica

8,866 Views

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09:29 min

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September 11, 2017

DOI:

10.3791/56134-v

DOI:
10.3791/56134-v

•

09:29 min
September 11, 2017

•

4 Views
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1Department of Anesthesiology,University of Texas Medical Branch

Summary

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Descriviamo l'uso di laser capture microdissection per ottenere campioni di popolazioni distinte delle cellule dalle regioni differenti del cervello per l'analisi del gene e microRNA. Questa tecnica permette lo studio di effetti differenziali del trauma cranico in regioni specifiche del cervello del ratto.

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Cite this Article

Weisz, H. A., Boone, D. R., Sell, S. L., Hellmich, H. L. Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury. J. Vis. Exp. (127), e56134, doi:10.3791/56134 (2017).

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