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Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury
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神経科学
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Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

DOI:
10.3791/56134-v

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09:29 min

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September 11, 2017

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, , ,
1Department of Anesthesiology,University of Texas Medical Branch

章

  • 00:05標題
  • 00:59Region Identification and Sectioning
  • 04:01Staining Protocol
  • 04:50Stereotactic Atlas Guided Laser Capture Microdissection with the LCM System
  • 07:36Results: LCM of Traumatic Brain Injury affected Brain Regions
  • 08:55Conclusion

概要

自動翻訳

自動翻訳

We describe the use of laser capture microdissection to obtain samples of distinct cell populations from different brain regions for gene and microRNA analysis. This technique allows the study of differential effects of traumatic brain injury in specific regions of the rat brain.

タグ

Stereotactic AtlasLaser Capture MicrodissectionTraumatic Brain InjuryFrontal CortexHippocampusMolecular AnalysisTranscriptional AnalysisCryostatOptimal Cutting Temperature MediumBrain RegionsCell Types

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