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使用快速、简单且便宜的 AWESAM 协议培养在体内像小鼠星形胶质细胞

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

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使用快速、简单且便宜的 AWESAM 协议培养在体内像小鼠星形胶质细胞

DOI:

10.3791/56092-v

•

07:56 min

•

January 10, 2018

•

Anne C. Wolfes^2,3, Camin Dean

¹Chemical Biology, Chemistry Research Laboratory,University of Oxford, ²Department of Physiology, Anatomy, and Genetics,University of Oxford, ³Trans-synaptic signaling,European Neuroscience Institute

Capítulos

00:05Título
00:39Rat or Mouse Brain Dissection
02:50Tissue Digestion and Dissociation
04:29Passaging Cells
06:24Results: Spontaneous Ca²⁺ Signaling in Astrocytes
07:12Conclusion

Summary

Tadução automática

这里描述的 AWESAM 协议是最理想的培养小鼠星形胶质细胞与其他脑神经干细胞的快速, 简单, 便宜的方式。AWESAM 星形胶质细胞展示自发 Ca^{2 +}信号, 形态学和基因表达谱类似于星形胶质细胞在体内。

Tags

In Vivo-like Astrocytes AWESAM Protocol Murine Astrocyte Culture Astrocyte Monocultures Astroglibiology Astrocyte Morphology Astrocyte MRNA And Protein Expression Astrocyte Calcium Fluctuations Cortical Astrocyte Isolation Hippocampal Astrocyte Isolation Trypsin-EDTA Treatment

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