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生産、結晶化、および人間 IKK1/α の構造決定へのガイド
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JoVE Journal Bioquímica
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
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生産、結晶化、および人間 IKK1/α の構造決定へのガイド

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11:27 min

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November 02, 2018

DOI:

10.3791/56091-v

DOI:
10.3791/56091-v

•

11:27 min
November 02, 2018

•

1 Views
, , ,
1Department of Chemistry & Biochemistry,University of California, San Diego, 2Department of Biophysics,Bose Institute

Summary

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IκB キナーゼ 1/α (IKK1/α CHUK) は主に転写因子の NF-κ B の活性化を介して細胞活動の無数に関係するセリン/スレオニン蛋白質キナーゼ。ここでは、生産、この蛋白質の結晶構造解析に必要な主な手順について述べる。

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Polley, S., Huang, D., Biswas, T., Ghosh, G. A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α. J. Vis. Exp. (141), e56091, doi:10.3791/56091 (2018).

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