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人的 ikkknα的生产、结晶和结构测定指南
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JoVE Journal Bioquímica
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
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人的 ikkknα的生产、结晶和结构测定指南

8,985 Views

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11:27 min

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November 02, 2018

DOI:

10.3791/56091-v

DOI:
10.3791/56091-v

•

11:27 min
November 02, 2018

•

1 Views
, , ,
1Department of Chemistry & Biochemistry,University of California, San Diego, 2Department of Biophysics,Bose Institute

Summary

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b 激酶 1/α (ikkniα-chuk) 是一种 ser/thr 蛋白激酶, 主要通过激活 nf-b 转录因子参与无数的细胞活动。在这里, 我们描述了生产和晶体结构测定这种蛋白质所需的主要步骤。

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Polley, S., Huang, D., Biswas, T., Ghosh, G. A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α. J. Vis. Exp. (141), e56091, doi:10.3791/56091 (2018).

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