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Pesquisa do Câncer
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Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

DOI:
10.3791/56081-v

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10:51 min

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August 23, 2017

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, , , , ,
1Molecular Medicine Division,Translational Genomics Research Institute, 2Agilent Technologies

Capítulos

  • 00:05Título
  • 00:51Pancreatic Cancer 3D Spheroid Magnetic Bioprinting
  • 03:473D Pancratic Tumor Spheroids Extracellular Flux Analysis
  • 07:34Results: Representative Spheroid Growth and Functionality Analyses
  • 09:37Conclusion

Summary

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Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.

Tags

3D SpheroidCo-culturePancreatic Cancer CellsFibroblastsMetabolic AssayExtracellular Flux AnalysisTumor MicroenvironmentCell MagnetizationNanoshuttleMagnetic Spheroid Drive

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